Quasispecies nature of the genome of Grapevine fanleaf virus

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Quasispecies nature of the genome of Grapevine fanleaf virus.

Genetic diversity was characterized in 14 isolates of Grapevine fanleaf virus (GFLV) recovered from grapevine (Vitis vinifera). Virions were collected by immunocapture, and a 1557 bp fragment containing part of the viral coat protein gene and part of the untranslated region to its 3' side was amplified by RT-PCR. Sequence variation among isolates was characterized by restriction fragment length...

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The Occurrence of Grapevine Fanleaf Virus in Washington State Vineyards

Grapevine degeneration in grapevines caused by Grapevine fanleaf virus (GFLV) has been documented in many viticultural regions worldwide (Andret-Link et al. 2004). It is one of the major economically important virus diseases affecting the longevity of grapevines and reducing the fruit yield and fruit quality. Infected grapevines show a range of foliar symptoms consisting of leaf deformation, ye...

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Nanobody‐mediated resistance to Grapevine fanleaf virus in plants

Since their discovery, single-domain antigen-binding fragments of camelid-derived heavy-chain-only antibodies, also known as nanobodies (Nbs), have proven to be of outstanding interest as therapeutics against human diseases and pathogens including viruses, but their use against phytopathogens remains limited. Many plant viruses including Grapevine fanleaf virus (GFLV), a nematode-transmitted ic...

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Isolation of the Gene Coding for Movement Protein from Grapevine Fanleaf Virus

A pair of degenerate primers, GMPF1 and GMPR1, was designed on the basis of alignment of previously reported Grapevine fanleaf virus (GFLV) movement protein (MP) nucleotide sequences from Iran and other parts of the world. cDNA was synthesized by the use of Oligo d(T)18 from total RNA extraction from each diseased grapevine leaf sample and subjected to polymerase chain reaction (PCR) with the d...

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Genome Diversity and Intra- and Interspecies Recombination Events in <italic>Grapevine fanleaf virus</italic>

Mekuria, T. A., Gutha, L. R., Martin, R. R., and Naidu, R. A. 2009. Genome diversity and intraand interspecies recombination events in Grapevine fanleaf virus. Phytopathology 99:1394-1402. Grapevine fanleaf virus (GFLV) was documented in self-rooted vines of four grapevine (Vitis vinifera) cultivars in eastern Washington. GFLV was found as mixed infection in cvs. Pinot Noir, Chardonnay, and Cab...

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ژورنال

عنوان ژورنال: Journal of General Virology

سال: 2001

ISSN: 0022-1317,1465-2099

DOI: 10.1099/0022-1317-82-7-1791